5-HTP(5-Hydroxytryptophan) ELISA Kit

SKU: ELK8124

Size:96T

Reactivity:General

Alternative Names:Oxitriptan; Cincofarm; Levothym; Levotonine; Oxyfan; Telesol; Tript-OH; Triptum

Assay Type:Competitive Inhibition

Sensitivity:1.74 ng/mL

Standard:400 ng/mL

Detection range:6.25-400 ng/mL

Sample type:serum, plasma and other biological fluids

Assay length:2h

Research Area:Enzyme & Kinase;

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-HTP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-HTP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-HTP in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: