2 × Phanta Flash Master Mix (Dye Plus) is a new generation superior enzyme based on Phanta Flash Super-Fidelity DNA Polymerase. Through directed optimization of Phanta DNA Polymerase,Phanta Flash Super-Fidelity DNA Polymerase has the characteristics of rapid amplification (4 - 5 sec/kb) while maintaining high fidelity and yield. Matched with optimized buffer system, this kit can achieve high amplification specificity. And it has excellent compatibility with crude samples, templates with uracil and GC-rich system (primer/template). This kit contains two types of monoclonal antibodies that inhibit the 5'→3' polymerase activity and 3'→5' exonuclease activity at room temperature, enabling it to perform hot start PCR with great specificity. It contains all required reaction components (Phanta Flash Super-Fidelity DNA Polymerase, dNTP and optimized buffer), except primers and templates, thereby simplifying the operation process and improving the detection throughput and repeatability. This kit contains loading buffer, which can be directly spotted for electrophoresis after the reaction. Amplification will generate blunt-ended products, which are compatible with ClonExpress kits (Vazyme #C112/C113/C115) and TOPO cloning kit.
