Library amplification and quantification are essential steps in next-generation sequencing to ensure there is enough material for downstream sequencing and that libraries are of sufficient quality. Library amplification involves selectively replicating DNA fragments within a library to generate sufficient genetic information that can then be quantified. This step results in increased material quantity, uniform representation, generation of clonal copies, and adapter incorporation. Library quantification is then used to determine the concentration and quality of the prepared DNA or RNA libraries. Accurate quantification is crucial for ensuring that the appropriate amount of material is loaded onto the sequencing platform and for optimizing the efficiency of the sequencing reaction. This step enables the optimization of sequencing input, quality control, pooling of libraries, and normalization.
