Recombinant Human GDNF Protein

SKU: PKSH032488

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: GDNF

Target Synonym: ATF;Astrocyte-Derived Trophic Factor;GDNF;Glial Cell Line-Derived Neurotrophic Factor;hGDNF

Research Areas: Neuroscience;Developmental Biology

Conjugation:

Target Species: Human

Expression Host: E.coli

Application: Cell culture

Fusion tag: C-His

UNIProt ID: P39905

Accession: P39905

Background: Glial Cell Line-Derived Neurotrophic Factor (GDNF) is a disulfide-linked homodimeric glycoprotein that belongs to the TGF-β superfamily. It has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells. Mutations in this gene may be associated with Hirschsprung disease.

Concentration:

Activity: Measure by its ability to induce proliferation in SH-SY5Y cells. The ED₅₀ for this effect is <10 ng/mL.

Sequence: Ser78-Ile211

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 20 mM sodium citrate, 0.2 M NaCl, pH 3.5.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.

Calculated MW: 16.0 kDa

ObservedMW: 16 kDa