Recombinant Human CD40L/TNFSF5 Protein (His Tag)

SKU: PKSH033726

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Immune Checkpoint Proteins

Abbreviation: CD40L;TNFSF5

Target Synonym: CD154;CD40 Ligand;CD40-L;CD40L;CD40LG;HIGM1;IGM;IMD3;T-BAM;T-Cell Antigen Gp39;TNF-Related Activation Protein;TNFSF5;TRAP;Tumor Necrosis Factor Ligand Superfamily Member 5;hCD40L

Research Areas: Cardiovascular;immunology

Conjugation:

Target Species: Human

Expression Host: HEK293 Cells

Application:

Fusion tag: N-His

UNIProt ID: P29965

Accession: P29965

Background: CD40 Ligand (CD40LG) is a type II transmembrane glycoprotein that belongs to the TNF superfamily. Like other TNF superfamily members; CD40LG exists as a trimer in membrane bound and soluble form; both of which are bioactive. CD40LG is a ligand for CD40; its ligation also initiates signal transduction in CD40LG expressing cells. CD40LG is a differentiation antigen that is expressed on the surface of T-cells. It stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40LG has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma; TNF-alpha; and IL2.

Concentration:

Activity: Not validated for activity

Sequence: Glu108-Leu261

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, 1mM EDTA, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 19.1 kDa

ObservedMW: 19-22 kDa