Phospho-SMAD3 (Ser425) Monoclonal Antibody

SKU: E-AB-51043

Size: 200μL

Storage: Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping: The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.

Exp date: 12 months

Category ID_II: Primary Antibodies

Category ID_III: Acetyl/Phospho/Methyl Antibodies

Abbreviation: Phospho-SMAD3 (Ser425)

Target Synonym: DKFZP586N0721;DKFZp686J10186;hMAD 3;hMAD-3;hSMAD3;HSPC193;HST17436;JV15 2;JV15-2;JV152;LDS1C;LDS3;MAD (mothers against decapentaplegic Drosophila) homolog 3;MAD homolog 3;Mad homolog JV15 2;Mad protein homolog;MAD;mothers against decapentaplegic homolog 3;Mad3;MADH 3;MADH3;MGC60396;Mothers against decapentaplegic homolog 3;Mothers against DPP homolog 3;SMA and MAD related protein 3;SMAD 3;SMAD;SMAD family member 3;SMAD;mothers against DPP homolog 3;Smad3;SMAD3

Research Areas: Cancer;Epigenetics and Nuclear Signaling;Metabolism;Signal Transduction;Stem Cells

Conjugation: Unconjugated

Host: Mouse

Species reactivity: Human;Mouse;Rat

Application: IHC-p

Isotype: IgG

Clonality: Monoclonal

Clone NO.: Clone:4C5

UNIProt ID: P84022

Accession:

Background: Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Concentration: 1 mg/mL

Immunogen: Synthetic Peptide of Phospho-Smad3 (Ser425)

Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.

Purification method: Protein A purification

Dilution: IHC 1:50-300

Calculated MW:

ObservedMW: