VD(Vitamin D) ELISA Kit

SKU: ELK7984

Size:96T

Reactivity:General

Alternative Names:VD; VD1; Lamisterol; Vitamin D; Vitamin D1; Lumisterol

Assay Type:Competitive Inhibition

Sensitivity:2.18 ng/mL

Standard:400 ng/mL

Detection range:6.25-400 ng/mL

Sample type:Serum, plasma and other biological fluids

Assay length:2h

Research Area:Metabonomics

Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with VD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to VD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VD in the samples is then determined by comparing the OD of the samples to the standard curve.

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