TUNEL In Situ Apoptosis Kit (HRP-DAB Method)

SKU: E-CK-A331

Size:100Assays

Storage:This product can be stored at -20°C for 12 months with shading light.

Shipping:Gel Packs

Exp date:12 months

Category ID_I:Apoptosis and Cell Health Detection

Category ID_II:Cell Apoptosis

Category ID_III:TUNEL Assay Kits

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Kit Type:Apoptosis/DNA Fragmentation

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Detection Instrument:Light microscope

Tested Sample Types:Paraffin section;frozen section;cell slide

Assay Time:3 hours

Detection Type:Colorimetric method

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Test Principle:When cells undergo apoptosis, endonuclease enzymes are activated to cleave the genomic DNA between nucleosomes. When genomic DNA is cleaved, the exposed 3'-OH can be added with a biotinylated dUTP (Biotin-dUTP) due to the catalysis of Terminal Deoxynucleotidyl Transferase (TdT), followed by the binding of horseradish Peroxidase-labeled Streptavidin (Streptavidin-HRP). Thereafter, through the HRP catalysis, the apoptotic cells are marked by DAB, which can be detected by ordinary microscopy. This is the principle of TUNEL (TdT-mediated dUTP Nick-End Labeling) for detecting apoptosis.

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