TransScript® II Green One-Step qRT-PCR SuperMix is designed for one-step qRT-PCR with high sensitivity, high synthesis efficiency and high amplification efficiency. This kit firstly synthesizes first-strand cDNA with RNA as templates using reverse gene-specific primers, and then performs qPCR with the synthesized cDNA as templates using both forward and reverse gene-specific primers to achieve one step from reverse transcription to qPCR in a single tube.
Highlights
• TransScript® II Green One-Step RT/RI Enzyme Mix is used to efficiently synthesize first-strand cDNA from RNA and 2×PerfectStartTM Green One-Step qPCR SuperMix is used for qPCR, enabling simple procedures to minimize the chance of contamination.
• High sensitivity and high specificity to ensure accurate results.
Applications
• High-copy and low-copy gene detection
• Detection of RNA virus or trace amounts of RNA
Storage
at -20°C away from light for two years
Shipping
Dry ice (-70 ℃)
| 100rxn x 20ul | 400rxn x 20ul | 1000rxn x 20ul | |
| Component | AQ311-01 | AQ311-02 | AQ311-03 |
| TransScript® II Green One-Step RT/ RI Enzyme Mix | 40 μl | 160 μl | 400 μl |
| PerfectStart® Green One-Step qPCR SuperMix (2×) | 1 ml | 4×1 ml | 10×1 ml |
| Passive Reference Dye (50×) | 40 μl | 160 μl | 400 μl |
| RNase-free Water | 1 ml | 4×1 ml | 10×1 ml |
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