TransNGS® ATAC-Seq Library Prep Kit for Illumina®

SKU: KP171-01

Product Details

TransNGS^® ATAC-Seq Library Prep Kit for Illumina^® is developed for the Illumina high-throughput sequencing platform and is suitable for constructing ATAC libraries from mammalian cells. The resulting libraries can be used for Single-Read or Paired-End sequencing. This kit utilizes a novel transposase enzyme technology for library construction targeting chromatin open regions, with experiments completed in only 3 hours. It requires minimal cell input, providing high-resolution sequencing profiles with starting cell amounts ranging from 50 to 50,000 cells.

Features

· Time saving and simple operation.

· Minimal cell input requirement. Library construction can be completed with as few as 50 cells.

Applications

Preparing chromatin open region fragments into short fragment libraries suitable for the Illumina high-throughput sequencing platform.

Storage

at -20℃ for one year.

Shipping

Dry ice (-70℃)

Product Composition

Component	KP171-01(12 rxns)	KP171-02(96 rxns)
Cell Lysis Buffer	24 μl	192 μl
Lysis Enhancer	12 μl	96 μl
4×Insertion Buffer	90 μl	720 μl
Insertion Enhancer 1	12 μl	96 μl
Insertion Enhancer 2	12 μl	96 μl
Tn5-50 Enzyme Mix	48 μl	384 μl
4×Tn5 Digestion Buffer	120 μl	960 μl
TransNGS ATAC-Seq Library Amplification SuperMix I（2×）	300 μl	4×600 μl
TransNGS ATAC-Seq Library Amplification SuperMix II（2×）	300 μl	4×600 μl
Library Elution Buffer	600 μl	5 ml
Nuclease-free Water	1 ml	2 × 5 ml
Tn5 Storage Buffer	600 μl	5 ml