Trans110 Chemically Competent Cell

SKU: CD311

Description	Trans110 Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 106 cfu/μg DNA (tested by pUC19 plasmid DNA).
Genotype	rpsL (StrR) thr leu thi-1 lacY galK galT ara tonA tsx dam dcm supE44 Δ(lac-proAB) /F′ [traD36proAB lacIq lacZΔM15]
Features	• High transformation efficiency: >106 cfu/μg ( pUC19 DNA). • Unmethylated DNA due to dam-/dcm-. • StrR.
Storage	at -70 °C for six months
Notes	Higher efficiency transformation can be achieved by transforming cells immediately following thawing. Avoid repeated thawing. Gentle handling is required for the entire procedure.

References:

•

Literature	Journal	IF	Author	Date	Link
MAPK signaling pathway alters expression of midgut ALP and ABCC genes and causes resistance to Bacillus thuringiensis Cry1Ac toxin in diamondback moth	PLoS Genetics	8.167	Zhaojiang Guo?? et al	2015 Apr	The original link
Bacillus thuringiensis subsp. sichuansis strain MC28 produces a novel crystal protein with activity against Culex quinquefasciatus larvae	World Journal of Microbiology and Biotechnology	1.262	Peng Guan, et al. State Key Laboratory of Hybrid R	2014 Apr	The original link
Cloning and characterisation of a novel cry1H gene effective against lepidopterous larvae from a Bacillus thuringiensis strain	Biocontrol Science and Technology	0.848	Yu Z, et al. Sichuan agricultural university	2015	The original link