Trans1-Blue Chemically Competent Cell

SKU: CD401-02

Description	Trans1-Blue Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 108 cfu/μg DNA (tested by pUC19 plasmid DNA). The competent cell is resistant to tetracycline (TetR).
Genotype	recA1 endA1 gyrA96 thi-1 hsdR17 supE44 (rk-,mk+), relA1 lac [F' proAB lacIqZΔM15: Tn10 (TetR)]
Features	• High transformation efficiency: >108 cfu/μg ( pUC19 DNA). • TetR. • Blue/white selection.
Storage	at -70 °C for six months
Notes	Higher efficiency transformation can be achieved by transforming cells immediately following thawing. Avoid repeated thawing. Gentle handling is required for the entire procedure.

References:

Literature	Journal	IF	Author	Date	Link
The N-Terminal GH10 Domain of a Multimodular Protein from Caldicellulosiruptor bescii Is a Versatile Xylanase/??-Glucanase That Can Degrade Crystalline Cellulose	Applied and Environmental Microbiology	3.668	Xianli Xue, et al.	2015 Mar	The original link
Controlling microbial PHB synthesis via CRISPRi	applied microbiology and biotechnology	3.42	Dan Li,et al.	2017May	The original link
Molecular cloning and characterization of DXS and DXR genes in the terpenoid biosynthetic pathway of Tripterygium wilfordii	international journal of molecular sciences	3.257	Tong Y, et al. School of Traditional Chinese Medic	2015 Oct	The original link
Metabolic engineering of an E. coli ndh knockout strain for PHB production from mixed glucose-xylose feedstock	journal of chemical technology and biotechnology	3.1349	Guangxin Huo,et al.	2017May	The original link
Engineering Escherichia coli for poly-(3-hydroxybutyrate) production guided by genome-scale metabolic network analysis	enzyme and microbial technology	2.502	Yangyang Zheng,et al.	2017Nov	The original link