TOP1MT Antibody - Cat. #: CSB-PA853372LA01HU

SKU: CSB-PA853372LA01HU

Size :50ug

Clone Number:

Aliases:TOP1MT antibody; DNA topoisomerase I antibody; mitochondrial antibody; TOP1mt antibody; EC 5.6.2.1 antibody

Product Type:Polyclonal Antibody

Immunogen Species:Homo sapiens (Human)

UniProt ID:Q969P6

Immunogen:Recombinant Human DNA topoisomerase I, mitochondrial protein (270-400AA)

Raised in:Rabbit

Species Reactivity:Human

Tested Applications:ELISA, IHC; Recommended dilution: IHC:1:500-1:1000

Background:Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3\\\'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5\\\'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5\\\'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).

Clonality:Polyclonal

Isotype:IgG

Purification Method:>95%, Protein G purified

Conjugate:Non-conjugated

Buffer:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form:Liquid

Stroage:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names:TOP1MT

Research Areas:Epigenetics and Nuclear Signaling