The picture shows the comparison between ssDNA 50 Ladder and single-stranded DNA with lengths of 49, 74, and 145 bases on 10% PAGE denaturing gel.
The ssDNA 50 Ladder is a single-stranded DNA ruler in increments of 50 bases. The shortest band corresponds to 50 nt, and the longest band exceeds 1000 nt. Samples were stored in 100 uL buffer containing: 50 mM HEPES (pH ~7), 100 mM NaCl, 2 mM ZnCl2, 2 mM EDTA. The total DNA concentration is approximately 20 ng/uL. ssDNA 50 Ladder is suitable for running denaturing PAGE gels.
Recommended usage method: Mix 3-5 uL ssDNA 50 Ladder sample with denatured Loading Buffer and load the sample. After gel running, soak and stain with SYBR Gold Nucleic Acid Gel Stain or Cyber Gold Stain for 10-20 minutes. The 5' end of ssDNA 50 Ladder contains -PO43-, and the 5' end can be labeled with fluorescence or 32P after removing this group. Instructions and Precautions: SYBR Gold Nucleic Acid Gel Stain or Cyber Gold Stain has the best effect on binding single-stranded nucleic acids. It is strongly recommended to use SYBR Gold or Cyber Gold staining to obtain the best gel pattern.
The ssDNA 50 Ladder is a single-stranded DNA ruler in increments of 50 bases. The shortest band corresponds to 50 nt, and the longest band exceeds 1000 nt. Samples were stored in 100 uL buffer containing: 50 mM HEPES (pH ~7), 100 mM NaCl, 2 mM ZnCl2, 2 mM EDTA. The total DNA concentration is approximately 20 ng/uL. ssDNA 50 Ladder is suitable for running denaturing PAGE gels.
Recommended usage method: Mix 3-5 uL ssDNA 50 Ladder sample with denatured Loading Buffer and load the sample. After gel running, soak and stain with SYBR Gold Nucleic Acid Gel Stain or Cyber Gold Stain for 10-20 minutes. The 5' end of ssDNA 50 Ladder contains -PO43-, and the 5' end can be labeled with fluorescence or 32P after removing this group. Instructions and Precautions: SYBR Gold Nucleic Acid Gel Stain or Cyber Gold Stain has the best effect on binding single-stranded nucleic acids. It is strongly recommended to use SYBR Gold or Cyber Gold staining to obtain the best gel pattern.