The picture shows the comparison between ssDNA 20 Ladder and single-stranded DNA with lengths of 35 and 89 bases respectively on 15% PAGE denaturing gel.
ssDNA 20 Ladder is a single-stranded DNA ruler with 20 base increments, including a total of 10 bands of 20, 40, 60, 80, 100, 120, 140, 160, 220, and 3100 nt. Samples were stored in 100 uL buffer containing: 25 mM HEPES (pH ~7), 50 mM NaCl, 1 mM ZnCl2, 1 mM EDTA. The total DNA concentration is approximately 50 ng/uL. ssDNA 20 Ladder is suitable for running denaturing PAGE gels, and the staining effect is best with SYBR Gold Nucleic Acid Gel Stain or Cyber Gold Stain.
Recommended usage method: Mix 3-5 uL ssDNA 20 Ladder sample with denatured Loading Buffer and load the sample. After gel running, soak and stain with SYBR Gold Nucleic Acid Gel Stain or Cyber Gold Stain for 10-20 minutes. The 5' end of ssDNA 20 Ladder contains -PO43-, and the 5' end can be labeled with fluorescence or 32P after removing this group. Instructions and Precautions: SYBR Gold Nucleic Acid Gel Stain or Cyber Gold Stain has the best effect on binding single-stranded nucleic acids. It is strongly recommended to use SYBR Gold or Cyber Gold staining to obtain the best gel pattern.