Simian Lptn/LTN/XCL1 (Lymphotactin) ELISA Kit

SKU: ELK11694

Size:96T

Reactivity:Simian

Alternative Names:Xcl1 ; Lptn ; Ltn ; Scyc1 ; Lymphotactin ; C motif chemokine 1 ; Cytokine SCM-1 ; Lymphotaxin ; Small-inducible cytokine C1

Assay Type:Sandwich

Sensitivity:0.12 ng/mL

Standard:25 ng/mL

Detection range:0.39-25 ng/mL

Sample type:Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay length:3.5h

Research Area:Cytokine;Tumor immunity;Infection immunity;Dermatology;

Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian Lptn/LTN/XCL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian Lptn/LTN/XCL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian Lptn/LTN/XCL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian Lptn/LTN/XCL1 in the samples is then determined by comparing the OD of the samples to the standard curve.

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