GeneBio Systems

SAA ELISA kit (Bovine)

Name: SAA ELISA kit (Bovine)
Brand: GeneBio Systems
SKU: SEA885Bo
Price: 1587.60 CAD
Availability: InStock

SKU:SEA885Bo

$1,587.60 CAD

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Quantity

Size: 96Tests

# of Times Cited in literature: 10

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: SAA

Target Full Name: Serum Amyloid A

Alternative Names: SA-A; SAA1; PIG4; TP53I4; Amyloid protein A; Amyloid fibril protein AA; Serum amyloid A-1 protein

Target Species: Bovine

Uniprot: P35541

Gene ID: 506412

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Bovine SAA / Serum Amyloid A

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 6.25-400ng/mL

Sensitivity: 2.72ng/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serum Amyloid A (SAA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serum Amyloid A (SAA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Serum Amyloid A (SAA). No significant cross-reactivity or interference between Serum Amyloid A (SAA) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serum Amyloid A (SAA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serum Amyloid A (SAA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serum Amyloid A (SAA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Serum Amyloid A (SAA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Infection immunity;Kidney biomarker;Hepatology;

References Citing This Product: EFFECT OF UTERINE IMMUNOMODULATION ON SERUM AMYLOID-A CONCENTRATION AND CONCEPTION RATE IN CYCLIC NON BREEDING COWS

Acute phase response in lame crossbred dairy cattle

Influence of yeast culture and feed antibiotics on ruminal fermentation and site and extent of digestion in beef heifers fed high grain rations

Using ruminally protected and nonprotected active dried yeast as alternatives to antibiotics in finishing beef steers: growth performance, carcass traits, blood …

Metabolism and immune status during transition period influences the lactation performance in Zebu (Bos indicus () cows

The predictive value of joint detection of serum amyloid protein A, PCT, and Hs-CRP in the diagnosis and efficacy of neonatal septicemia.

Evaluation of infrared thermography compared to rectal temperature to identify illness in early postpartum dairy cows

Ruminally protected and unprotected Saccharomyces cerevisiae fermentation products as alternatives to antibiotics in finishing beef steers

Serum amyloid A as an marker of cow֨ s mastitis caused by Streptococcus sp.

Peripheral concentrations of metabolic and inflammatory indicators during transition period and their relationship with postpartum clinical endometritis in dairy …

Processing Index of Barley Grain and Dietary Undigested Neutral Detergent Fiber Concentration Affected Chewing Behavior, Ruminal pH and Total Tract Nutrient?¡