REG3g ELISA kit (Mouse)

SKU: SEE676Mu

Size: 96Tests

# of Times Cited in literature: 4

Prepare Time: 1-3 days(please inquire for mutiple units)

Target Name: REG3g

Target Full Name: Regenerating Islet Derived Protein 3 Gamma

Alternative Names: REG-III; PAP1B; PAPIB; UNQ429; Pancreatitis-associated protein 1B; Regenerating islet-derived protein III-gamma

Target Species: Mouse

Uniprot: O09049

Gene ID: 19695

Featured Series: SE kit

Featured Series Function: Detects protein (regular version)

Specificity: Reactive with Mouse REG3g / Regenerating Islet Derived Protein 3 Gamma

Method: Colormetric

Detection principle: Double-antibody Sandwich

Detection range: 31.2-2,000pg/mL

Sensitivity: 12.1pg/mL

Assay Time: 3h

Sample Size: 100uL

Recommended/Predicted Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids

Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%

Reproducibility test menthod: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Regenerating Islet Derived Protein 3 Gamma (REG3g) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Regenerating Islet Derived Protein 3 Gamma (REG3g) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100

Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)

Shelf-life: 12 months

Specificity: This assay has high sensitivity and excellent specificity for detection of Regenerating Islet Derived Protein 3 Gamma (REG3g). No significant cross-reactivity or interference between Regenerating Islet Derived Protein 3 Gamma (REG3g) and analogues was observed.

Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary: 1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regenerating Islet Derived Protein 3 Gamma (REG3g). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Regenerating Islet Derived Protein 3 Gamma (REG3g). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Regenerating Islet Derived Protein 3 Gamma (REG3g), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Regenerating Islet Derived Protein 3 Gamma (REG3g) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Research Area: Infection immunity;

References Citing This Product: Hyperglycaemia inhibits REG3A expression to exacerbate TLR3-mediated skin inflammation in diabetes.

Enteric Delivery of Regenerating Family Member 3 alpha Alters the Intestinal Microbiota and Controls Inflammation in Mice With Colitis

Interleukin-6/Stat3 signaling has an essential role in the host antimicrobial response to urinary tract infection

Role of TLR5 in the Translocation and Dissemination of Commensal Bacteria in the Intestine after Traumatic Hemorrhagic Shock