Recombinant TCF7 Monoclonal Antibody

SKU: AN300696L

Size::50μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Recombinant Antibodies;Monoclonal Antibodies

Abbreviation:TCF7

Target Synonym:TCF;TCF7;TCF-1;TCF1;FLJ36364;MGC47735;OTTHUMP00000159391;T cell factor 1;T cell specific transcription factor 1;T-cell factor 1;T-cell specific;T-cell-specific transcription factor 1;TCF-7;TCF7 transcription factor 7;Transcription factor 7;Transcription factor 7 (T cell specific HMG box);Transcription factor-7

Research Areas:Epigenetics and Nuclear Signaling;Immunology;Stem Cells

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human;Rat

Application:WB

Isotype:IgG,κ

Clonality:Monoclonal;Recombinant

Clone NO.:10F12

UNIProt ID:P36402

Accession:

Background:2 series of isoforms, L and S, are produced by use of alternative promoter usage. Additional isoforms seem to exist,Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1.,induction:By TCF7L2 and CTNNB1.,sequence caution:Wrong choice of frame.,similarity:Belongs to the TCF/LEF family.,similarity:Contains 1 HMG box DNA-binding domain.,subunit:Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with AES, TLE1, TLE2, TLE3 and TLE4.,tissue specificity:Predominantly in T-cells. Also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium.

Concentration:0.2 mg/mL

Immunogen:Recombinant Human TCF7 protein

Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Purification method:Protein A

Dilution:WB 1:2000-1:10000

Calculated MW:42 kDa

ObservedMW:60 kDa