Recombinant Swine TNF alpha protein(His Tag)

SKU: PKSS000010

Size: 20μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Cytokines

Abbreviation: TNF alpha

Target Synonym: Cachectin;Cytotoxin;Differentiation-inducing factor (DIF);Necrosin;TNFSF2;TNS F1A

Research Areas:

Conjugation:

Target Species: Porcine

Expression Host: E.coli

Application: Cell culture

Fusion tag: C-His

UNIProt ID: P23563

Accession: P23563

Background: Tumor necrosis factor alpha (TNFα) is the prototypic ligand of the TNF superfamily. TNFα forms a homotrimer and functions by activating two types of receptors TNF-R1 (TNF receptor type 1,p55R) and TNF-R2 (TNF receptor type 2,p75R). TNFα is a pleiotropic cytokine that is capable to promote inflammation, to induce apoptotic cell death, and to inhibit tumorigenesis and viral replication. TNFα is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells.

Concentration:

Activity: Measure by its ability to induce cytotoxicity in PK15 cells in the presence of the actinomycin D.The ED₅₀ for this effect is <15 pg/mL.

Sequence: Leu 77-Leu 232

Purity: > 98 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.

Calculated MW: 18.1 kDa

ObservedMW: 19 kDa