Recombinant SARS-CoV S-trimer Protein (C-6His)

SKU: PKSV030289

Size: 500μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Viral Proteins

Abbreviation: Spike

Target Synonym: SARS-CoV S glycoprotein;SARS-CoV S-trimer Protein;SARS-CoV Spike glycoprotein;SARS-CoV Sprotein

Research Areas:

Conjugation:

Target Species: SARS

Expression Host: HEK293 Cells

Application: ELISA

Fusion tag: C-His

UNIProt ID: P59594

Accession: P59594

Background: The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain

Concentration:

Activity: Immobilized SARS-CoV S-trimer Protein-His(PKSV030289) at 5μg/ml (100 μl/well) can bind Human ACE-2- Fc(PKSR030492). The ED50 of Recombinant Human ACE-2- Fc(PKSR030492) is 33. 26 ng/ml.

Sequence: Ser14-Gln1190

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of PBS, pH7.4.

Reconstitution: Not Applicable

Endotoxin: Please contact us for more information.

Calculated MW: 134.9 kDa

ObservedMW: 170-220 kDa