Recombinant SARS-CoV-2 ORF3b protein

SKU: PDEV100011

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Viral Proteins

Abbreviation:ORF3b

Target Synonym:ORF3b;ORF3b protein

Research Areas:

Conjugation:

Target Species:SARS-CoV-2

Expression Host:E.coli

Application:

Fusion tag:N-SUMO & C-His

UNIProt ID:P0DTF1

Accession:P0DTF1

Background:SARS-CoV-2 ORF3b is homologous to SARS-CoV ORF3b, although the proposed protein is much shorter due to the presence of premature stop codons (Probable). Elicits strong specific antibody response (PubMed:32807944).

Concentration:

Activity:Not validated for activity

Sequence:Met1-Val22

Purity: > 90% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:18.2 kDa

ObservedMW: