Recombinant Saccharomyces cerevisiae Lanosterol 14-alpha demethylase(ERG11),partial

Recombinant Saccharomyces cerevisiae Lanosterol 14-alpha demethylase(ERG11),partial

CSB-EP006459SVG1
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Size: 200ug. Other sizes are also available. Please Inquire.

In Stock: No

Lead time: 10-20 working days

Research Topic: Others

Uniprot ID: P10614

Gene Names: ERG11

Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

AA Sequence: MSATKSIVGEALEYVNIGLS

Expression Region: 1-20aa

Sequence Info: Extracellular Domain

Source: E.coli

Tag Info: N-terminal GST-tagged

MW: 29.1 kDa

Alternative Name(s): CYPLI Cytochrome P450 51 Cytochrome P450-14DM Cytochrome P450-LIA1 Sterol 14-alpha demethylase

Relevance: Catalyzes C14-demethylation of lanosterol which is critical for ergosterol biosynthesis. It transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol.

Reference: "Primary structure of the P450 lanosterol demethylase gene from Saccharomyces cerevisiae."Kalb V.F., Woods C.W., Turi T.G., Dey C.R., Sutter T.R., Loper J.C.DNA 6:529-537(1987)

Purity: Greater than 90% as determined by SDS-PAGE.

Storage Buffer: Tris-based buffer,50% glycerol

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

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