Recombinant Rhesus Macaque Flt-3 Ligand

SKU: CD00046

Size:2 ug

Background:Flt-3 ligand (FL) is a recently identified hematopoietic cytokine whose activities are mediated by binding to the transmembrane glycoprotein Flt-3. Flt-3 was first discovered as a member of the class III subfamily of receptor tyrosine kinases (RTK) whose expression among hematopoietic cells was found to be restricted to highly enriched stem/progenitor cell populations. Additionally, class III RTKs include the receptors from SCF, M-CSF and PDGF. Not surprisingly, Flt-3 ligand is also structurally related to M-CSF and SCF. All three cytokines have been shown to exist both as type I transmembrane proteins and as soluble proteins. The predominant human FL isoform is a transmembrane protein that can undergo proteolytic cleavage to generate a soluble form of the protein. FL has been shown to synergize with a wide variety of hematopoietic cytokines to stimulate the growth and differentiation of early hematopoietic progenitors.

Research_area:Immunology

Purity:> 97 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Rhesus Macaque Fms-related Tyrosine Kinase 3 Ligand

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using human AML5 cells is less than 1. ng/ml, corresponding to a specific activity of > 1. × 16 IU/mg.

Synonyms:Flt-3 Ligand, Monkey

Endotoxin:Less than 1 EU/μg of rRhFlt-3L as determined by LAL method.

Storage:-20ºC

Sequence:TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVPSNLQD EELCGALWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQHP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPRSPGALEA TALTAPQRP

uniprot accession:Q95M3

Molecular weight:Approximately 18. kDa, a single non-glycosylated polypeptide chain containing 159 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered solution in PBS, pH 7.4.