Recombinant Rat SCF protein

SKU: CD00076

Size:2 ug

Background:Stem Cell Factor (SCF) which binds to the c-Kit receptor is produced by fibroblasts and endothelial cells. The soluble and transmembrane forms of the protein are formed by alternative splicing of the same RNA transcript and the presence of both soluble and transmembrane SCF is required for normal hematopoietic function. SCF plays an important role in hematopoiesis, spermatogenesis, and melanogenesis. In addition, it also promotes mast cell adhesion, migration, proliferation, and survival 3. Rat SCF shares 75 % - 90 % a.a. sequence identity with canine, feline, mouse, and human SCF. Furthermore, rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells.

Research_area:Immunology

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Rat Stem Cell Factor

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using Mouse MC/9-2 mast cells is less than 5 ng/ml, corresponding to a specific activity of > 2. × 15 IU/mg.

Synonyms:KITLG, Kitl, Mgf, SCF,Rat

Endotoxin:Less than 1 EU/μg of rRtSCF as determined by LAL method.

Storage:-20ºC

Sequence:MQEICRNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV THLSVSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVAC MEENAPKNVK ESLKKPETRN FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA

uniprot accession:P21581

Molecular weight:Approximately 18.5 kDa, a single non-glycosylated polypeptide chain containing 165 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.