Recombinant Rat OSM protein

SKU: CD00083

Size:2 ug

Background:Oncostatin-M (OSM) is a multifunctional cytokine that belongs to the Interleukin-6 subfamily. Among the family members, OSM is most closely related to leukemia inhibitory factor (LIF) and it in fact utilizes the LIF receptor in addition to its specific receptor in the human. A biologically active OSM receptor has been previously described that consists of a heterodimer of leukemia inhibitory factor receptor (LIFR) and gp130. OSM is synthesized by stimulated T-cells and monocytes. Furthermore, the effects of OSM on endothelial cells suggest a pro-inflammatory role for OSM and endothelial cells possess a large number of OSM receptors. Recombinant Mouse OSM contains 215 amino acids and has a molecular mass of 22.4 kDa. It has approximately 48 % and 72 % amino acid sequence identity with human and Mouse OSM.

Research_area:Immunology

Purity:> 96 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Rat Oncostatin-M

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using Mouse NIH-3T3 cells is less than 2. ng/ml, corresponding to a specific activity of > 5. × 15 IU/mg.

Synonyms:OSM, Rat

Endotoxin:Less than 1 EU/μg of rRtOSM as determined by LAL method.

Storage:-20ºC

Sequence:KRGCSSSSPK LLSQLKSQAN ITGNTASLLE PYILHQNLNT LTLRAACTEH PVAFPSEDML RQLSKPDFLS TVHATLGRVW HQLGAFRQQF PKIQDFPELE RARQNIQGIR NNVYCMARLL HPPLEIPEPT QADSGTSRPT TTAPGIFQIK IDSCRFLWGY HRFMGSVGRV FEEWGDGSRR SRRHSPLWAW LKGDHRIRPS RSSQSAMLRS LVPR

uniprot accession:Q65Z15

Molecular weight:Approximately 24.3 kDa, a single non-glycosylated polypeptide chain containing 214 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 2 × PBS, .1 % Tween-8.