Recombinant Rat Ly6/PLAUR domain-containing protein 3 (Lypd3) (Active)

SKU: O55162

Size: 100ug. Other sizes are also available.

Activity: Yes

Research Areas: Cancer

Uniprot ID: O55162

Gene Names: Lypd3

Alternative Name(s): Ly6/PLAUR domain-containing protein 3; GPI-anchored metastasis-associated protein C4.4A; Lypd3; C4.4a

Abbreviation: Recombinant Rat Lypd3 protein (Active)

Organism: Rattus norvegicus (Rat)

Source: Mammalian cell

Expression Region: 33-330aa

Protein Length: Full Length of Mature Protein

Tag Info: C-terminal 10xHis-tagged

Target Protein Sequence: LECYSCVQKADDGCSPHKMKTVKCGPGVDVCTEAVGAVESIHGQFSVAVRGCGSGIPGKNDRGLDLHGLLAFIQLQQCTEDRCNAKLNLTLRGLNPAGNESAYEHNGAECYSCMGLSREKCQGAMPPVVNCYNASGRVYKGCFDGNVTLTAANVTVSLPVRGCVQDEACTRDGVTGPGFTLSGSCCQGPRCNSDLRNKTYFSPRIPPLVLLPPPTTPAPSTRTQNSSSTTSTTAPTTATTTIKPTTVQASHTSSTHETEHEVIQEEGSHLSGGATGHQDRSNMGKFPEKGGAQIPSKG

MW: 32.6 kDa

Purity: Greater than 95% as determined by SDS-PAGE.

Endotoxin: Less than 1.0 EU/μg as determined by LAL method.

Biological_Activity: Measured by its binding ability in a functional ELISA.Immobilized Rat Lypd3 at 2 μg/mL can bind Anti-LYPD3 recombinant antibody(CSB-RA013263MA2HU). The EC50 is 3.515-3.992 ng/mL.

Form: Lyophilized powder

Buffer: Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance: Supports cell migration. May be involved in tumor progression.

Reference: Ly6 family member C4.4A binds laminins 1 and 5, associates with galectin-3 and supports cell migration. Paret C., Bourouba M., Beer A., Miyazaki K., Schnoelzer M., Fiedler S., Zoeller M. Int. J. Cancer 115: 724-733 (2005)

Function: