Recombinant Rat Interleukin-12 subunit beta/IL-12B protein (His tag)

SKU: PDMR100029

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:IL-12B

Target Synonym:NKSF;IL-12/IL-23 p;IL-12 subunit p;NK cell stimulatory factor chain;NK cell Stimulating Factor Chain;CLMF;CLMF p;IL12 p;CLMF2;Cytotoxic lymphocyte maturation factor 40 kDa subunit;Cytotoxic Lymphocyte Maturation Factor 40 kDa subunit (CLMF p40);IL12;IL-12 p40;IL12 p70;IL-12 p70;IL-12/IL-23 p40;NK cell Stimulating Factor Chain 2;p40;IL-12B;NKSF2;Interleukin-12 subunit beta;CLMF p40;IL-12 subunit p40;NK cell stimulatory factor chain 2;IL 12B;Il12b

Research Areas:Signal Transduction;epigenetics and nuclear signal;immunology;

Conjugation:

Target Species:Rat

Expression Host:HEK293 Cells

Application:

Fusion tag:C-His

UNIProt ID:E9PU71

Accession:E9PU71

Background:Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of pro-inflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.

Concentration:

Activity:Not validated for activity

Sequence:Met1-Ser335

Purity: > 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 1.0 EU/mg of the protein as determined by the LAL method.

Calculated MW:36.8 kDa

ObservedMW:42 kDa