Recombinant Rat IL-1A/ IL-1α Protein

SKU: PKSR030449

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Cytokines

Abbreviation: IL-1A;IL-1α

Target Synonym: interleukin;Hematopoietin;IL1F1hematopoietin;IL1F;IL-1F;alpha;BAF;Hematopoietin-1;IL1;IL1 alpha;IL-1 alpha;IL1-A;IL-1A;IL1-ALPHA;IL1F1;IL-1F1;IL1F1hematopoietin-1;interleukin 1;interleukin-1 alpha;LAF;LEM;preinterleukin 1 alpha;pro-interleukin-1-alpha;Il1a;FAF;Hematopoietin 1;IL 1 alpha;IL 1A;IL1;IL1 ALPHA;IL1F1;Interleukin 1 alpha;LAF;LEM;Preinterleukin 1 alpha;Il1a

Research Areas:

Conjugation:

Target Species: Rat

Expression Host: E.coli

Application:

Fusion tag: None

UNIProt ID: P16598

Accession: P16598

Background: Interleukin 1 (IL-1) is a name that designates two proteins, IL-1αand IL-1β, which are the products of distinct genes, but which show approximately 25% amino acid (aa) sequence identity and which recognize the same cell surface receptors. IL-1αand IL-1β are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17,000 Da. Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL-1α remains in the cytosol of cells, although there is evidence for a membranebound form of the precursor form of IL-1α. Although IL-1 production is generally considered to be a consequence of inflammation, evidence suggests that IL-1 is also temporally upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. In response to classic stimuli produced by inflammatory agents, infections or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cells is seen.

Concentration:

Activity: Not validated for activity

Sequence: Ser115-Ser270

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 17.9 kDa

ObservedMW: 16 kDa