Recombinant Rat IL-1 Beta protein

SKU: CD00112

Size:2 ug

Background:Interleukin-1beta (IL-1β) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1αand IL-1β binds to the same receptor and has similar but not identical biological properties. The mature rat IL-1β shares 90 % a.a. sequence identity with cotton rat and mouse and 65 % to 77 % with canine, human and rhesus IL-1β.

Research_area:Immunology

Purity:> 96 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Rat Interleukin-1 beta

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using Mouse D1S cells is less than .1 ng/ml, corresponding to a specific activity of > 1. × 17 IU/mg.

Synonyms:IL-1β, IL-1F2,IL1B,Rat

Endotoxin:Less than 1 EU/μg of rRtIL-1β as determined by LAL method.

Storage:-20ºC

Sequence:MVPIRQLHCR LRDEQQKCLV LSDPCELKAL HLNGQNISQQ VVFSMSFVQG ETSNDKIPVA LGLKGLNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRF VFNKIEVKTK VEFESAQFPN WYISTSQAEH RPVFLGNSNG RDIVDFTMEP VSS

uniprot accession:Q63264

Molecular weight:Approximately 17.3 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.