Recombinant Rat CINC-3/CXCL2 protein

SKU: CD00134

Size:5 ug

Background:Rat CXCL2 also named CINC-3, belongs to the CXC chemokine family. It is encoded by the gene CXCL2 and shares 90 % amino acid sequence with CXCL1/GROα. It is member of the intercrine alpha (chemokine C-X-C) subfamily of chemokines. This chemokine is expressed by cytokine-stimulated rat alveolar macrophages and fibroblasts. The functional receptor for CXCL2 has been identified as CXCR2. CXCL2 is chemotactic for polymorphonuclear leukocytes and hematopoietic stem cells. Similar to other GRO proteins, CXCL2 is potent neutrophil attractants and activators. In addition, it is also active toward basophils. The amino acid sequence of rat CXCL2 is 88 % identical to Mouse CXCL2.

Research_area:Immunology

Purity:> 98 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Rat Cytokine-induced Neutrophil Chemoattractant 3/CXCL2

Biological activity:Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using rat neutrophils is in a concentration range of 1-1 ng/ml.

Synonyms:CINC-3/CXCL2,Mip-2; Scyb2, Rat

Endotoxin:Less than 1 EU/μg of rRtCINC-3/CXCL2 as determined by LAL method.

Storage:-20ºC

Sequence:VVVASELRCQ CLTTLPRVDF KNIQSLTVTP PGPHCAQTEV IATLKDGHEV CLNPEAPLVQ RIVQKILNKG KAN

uniprot accession:P3348

Molecular weight:Approximately 7.9 kDa, a single, non-glycosylated polypeptide chain containing 73 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4.