Recombinant Rat CD73/NT5E Protein (His Tag)

SKU: PKSR030462

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: CD73;NT5E

Target Synonym: 5'-Nucleotidase;CD73;E5NT;NT5E;Nucleotidase;ecto-5'-nucleotidase

Research Areas:

Conjugation:

Target Species: Rat

Expression Host: HEK293 Cells

Application:

Fusion tag: C-His

UNIProt ID: P21588

Accession: P21588

Background: CD73, also known as Ecto-5'-nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor. The enzyme is expressed by most cell types. The 5 ’ -Nucleotidase activity of CD73 converts extracellular nucleoside-5 ’ -monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides.

Concentration:

Activity: Not validated for activity

Sequence: Trp29-Lys549

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 120mM NaCl, 4mM CaCl₂, 20% Glycerol, pH 7.5.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 59.0 kDa

ObservedMW: 70-90 kDa