Recombinant Rat BD-1 protein

SKU: CD00143

Size:5 ug

Background:Defensins (alpha and beta) are cationic peptides with antimicrobial activity against Gram-negative and Gram-positive bacteria, fungi, and enveloped viruses. They are 2-6 kDa proteins and take important roles in innate immune system. On the basis of their size and pattern of disulfide bonding, mammalian defensins are classified into alpha, beta and theta categories. β-Defensins are expressed on some leukocytes and at epithelial surfaces. They contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Because β-defensins are cationic peptides, they can therefore interact with the membrane of invading microbes, which are negative due to lipopolysaccharides (LPS) and lipoteichoic acid (LTA) found in the cell membrane. Especially, they have higher affinity to the binding site compared to Ca2+ and Mg2+ ions. Furthermore, they can affect the stability of the membrane. Additionally, they are not only have the ability to strengthen the innate immune system but can also enhance the adaptive immune system by chemotaxis of monocytes, T-lymphocytes, dendritic cells and mast cells to the infection site.

Research_area:Immunology

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Rat Beta-defensin 1

Biological activity:Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using CD34+ dendritic cells is in a concentration range of 1.-1. ng/ml.

Synonyms:BD-1, CDK4,BD1,Defb1,Rat

Endotoxin:Less than 1 EU/μg of rRtBD-1 as determined by LAL method.

Storage:-20ºC

Sequence:DQYRCLQNGG FCLRSSCPSH TKLQGTCKPD KPNCCRS

uniprot accession:O89117

Molecular weight:Approximately 4.1 kDa, a single non-glycosylated polypeptide chain containing 37 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 2 mM PB, 5 mM NaCl, pH 7..