Recombinant Rat 4-1BB/TNFRSF9/CD137 Protein(Fc Tag)

SKU: PDMR100066

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Immune Checkpoint Proteins

Abbreviation:4-1BB/TNFRSF9/CD137

Target Synonym:

Research Areas:

Conjugation:

Target Species:Rat

Expression Host:Mammalian

Application:

Fusion tag:C-Fc

UNIProt ID:Q4V895

Accession:Q4V895

Background:CD137 (also known as 4-1BB) is a surface co-stimulatory glycoprotein originally described as present on activated T lymphocytes , which belongs to the tumor necrosis factor (TNF) receptor superfamily. It is expressed mainly on activated CD4+ and CD8+ T cells , and binds to a high-affinity ligand (4-1BBL) expressed on several antigen-presenting cells such as macrophages and activated B cells. Upon ligand binding , 4-1BB is associated with the tumor necrosis factor receptor–associated factors (TRAFs) , the adaptor protein which mediates downstream signaling events including the activation of NF-kappaB and cytokine production. 4-1BB signaling either by binding to 4-1BBL or by antibody ligation delivers signals for T-cell activation and growth , as well as monocyte proliferation and B-cell survival , and plays an important role in the amplification of T cell-mediated immune responses. In addition , CD137 and CD137L are expressed in different Human primary tumor tissues , suggesting that they may influence the progression of tumors. Crosslinking of CD137 on activated T cells has shown promise in enhancing anti-tumor immune responses in murine models , and agonistic anti-CD137 antibodies are currently being tested in phase I clinical trials. Soluble forms of CD137 (sCD137) are generated by differential splicing. sCD137 can bind to CD137 ligand to antagonize the costimulatory activities of the membrane-bound CD137 and reduce T cell proliferation and IL-2 secretion.

Concentration:

Activity:Not validated for activity

Sequence:Thr24-Val188

Purity: > 90% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 1.0 EU/mg of the protein as determined by the LAL method.

Calculated MW:43.0 kDa

ObservedMW:55-60 kDa