Recombinant PPM1A/PP2CA Monoclonal Antibody

SKU: AN300501P

Size::

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Recombinant Antibodies;Monoclonal Antibodies

Abbreviation:PPM1A

Target Synonym:Pppm1a;Ppm1a;2310003C21Rik;2900017D14Rik;AI427932;AU017636;MMPa-2;MPPa-1

Research Areas:

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Mouse

Application:WB

Isotype:IgG

Clonality:Monoclonal

Clone NO.:8C5

UNIProt ID:P49443

Accession:

Background:Protein phosphatase 1A (PPM1A/PP2CA) is an enzyme belonging to the PP2C family of Ser/Thr protein phosphatases. Members of PP2C family are negative regulators of cell stress response pathways and the MAP kinases and MAP kinase kinases. It has also been demonstrated to inhibit the activation of p38 and JNK kinase cascades. PPM1A dephosphorylates and promotes nuclear export of TGFβ-activated Smad2/3. Ectopic expression of PPM1A abolishes TGFβ-induced antiproliferative and transcriptional responses, whereas depletion of PPM1A enhances TGFβ signaling in mammalian cells. It has been demonstrated that PPM1A/PP2CA, through dephosphorylation of Smad2/3, plays a critical role in terminating TGFβ signaling. Overexpression of PPM1A is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to cell apoptosis.

Concentration:1 mg/mL

Immunogen:Recombinant Mouse PPM1A/PP2CA Protein

Buffer:0.2 μm filtered solution in PBS

Purification method:Protein A

Dilution:WB 1:500-1:2000

Calculated MW:42 kDa

ObservedMW:45 kDa