Recombinant Porcine IL-1 Beta protein

SKU: CD00155

Size:2 ug

Background:Interleukin-1 beta (IL-1β) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1α and IL-1β binds to the same receptor and has similar but not identical biological properties. Recombinant porcine interleukin-1 beta is a 17 kDa protein containing 153 amino acid residues and it shares 63 % - 70 % a.a. sequence identity with canine, cotton rat, equine, feline, human, mouse, rat, and rhesus IL-1β.

Research_area:Immunology

Purity:> 95 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Porcine Interleukin-1 beta

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using Mouse D1S cells is less than 5. ng/ml, corresponding to a specific activity of > 2. × 15 IU/mg.

Synonyms:IL-1β, IL-1beta, IL1B,IL1B2,Porcine

Endotoxin:Less than 1 EU/ug of rPoIL-1β as determined by LAL method.

Storage:-20ºC

Sequence:ANVQSMECKL QDKDHKSLVL AGPHMLKALH LLTGDLKREV VFCMSFVQGD DSNNKIPVTL GIKGKNLYLS CVMKDNTPTL QLEDIDPKRY PKRDMEKRFV FYKTEIKNRV EFESALYPNW YISTSQAEQK PVFLGNSKGR QDITDFTMEV LSP

uniprot accession:P26889

Molecular weight:Approximately17.6 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in PBS, pH 7.4, 3 % trehalose.