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GeneBio Systems

Recombinant Phospho-BTK (Tyr551) Monoclonal Antibody

Recombinant Phospho-BTK (Tyr551) Monoclonal Antibody

AN302103L

$512.44 CAD
$512.44 CAD
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Size::50μL

Storage:Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.

Shipping:Ice bag

Exp date:12 months

Category ID_II:Primary Antibodies

Category ID_III:Monoclonal Antibodies;Recombinant Antibodies;Acetyl/Phospho/Methyl Antibodies

Abbreviation:Phospho-BTK (Tyr551)

Target Synonym:IMD;AGMX;PSCTK;AGMX1;AT;ATK;BPK;IMD1;PSCTK1;XLA;BTK;Bruton tyrosine kinase;AGMX1;ATK;BPK;Bruton tyrosine kinase;IMD1;PSCTK1;XLA

Research Areas:Signal Transduction;Immunology

Conjugation:Unconjugated

Host:Rabbit

Species reactivity:Human;

Application:FCM

Isotype:IgG,κ

Clonality:Monoclonal;Recombinant

Clone NO.:A827

UNIProt ID:Q06187

Accession:

Background:Bruton's tyrosine kinase (Btk) is a member of the Btk/Tec family of cytoplasmic tyrosine kinases. Like other Btk family members, it contains apleckstrin homology (PH) domain and Src homology SH3 and SH2 domains. Btk plays an important role in B cell development. Activation ofB cells by various ligands is accompanied by Btk membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane-localized Btk is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 andTyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinases, leading to autophosphorylation at Tyr223 withinthe SH3 domain, which is necessary for full activation. The activation of Btk is negatively regulated by PKCβ through phosphorylation of Btkat Ser180, which results in reduced membrane recruitment, transphosphorylation, and subsequent activation. The PKC inhibitory signal islikely to be a key determinant of the B cell receptor signaling threshold to maintain optimal Btk activity.

Concentration:1 mg/mL

Immunogen:Peptide. This information is proprietary to PTMab

Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.

Purification method:Protein A purified

Dilution:FCM 1:100

Calculated MW:

ObservedMW:

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