Recombinant Mycoplasma hyorhinis Variant surface antigen F (vlpF), partial

SKU: Q49538

Size: 100ug. Other sizes are also available.

Activity: Not tested

Research Areas: Others

Uniprot ID: Q49538

Gene Names: vlpF

Alternative Name(s): (VlpF prolipoprotein)

Abbreviation: Recombinant Mycoplasma hyorhinis vlpF protein, partial

Organism: Mycoplasma hyorhinis

Source: E.coli

Expression Region: 30-67aa

Protein Length: Partial

Tag Info: N-terminal 6xHis-KSI-tagged

Target Protein Sequence: CGQTNTDQSQQPGSGSGSGSGTSNGSGSTPTPEQGNNQ

MW: 18.9 kDa

Purity: Greater than 90% as determined by SDS-PAGE.

Endotoxin: Not test

Biological_Activity:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance: Responsible for the antigenic diversity for host adaptation. Expression in E.coli of a construct containing vlpD, vlpE, and vlpF yields antigenically distinguishable products corresponding to each gene.

Reference: "Increased structural and combinatorial diversity in an extended family of genes encoding Vlp surface proteins of Mycoplasma hyorhinis." Yogev D., Watson-Mckown R., Rosengarten R., Im J., Wise K.S. J. Bacteriol. 177: 5636-5643(1995)

Function: