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Updated Date: Stock Protein updated on 20170405
Research areas: Microbiology
Target / Protein: dps
Biologically active: Not Tested
Expression system: E.coli
Species of origin: Mycobacterium smegmatis
Delivery time: 3-7 business days
Uniprot ID: P0C558
AA Sequence: MTSFTIPGLSDKKASDVADLLQKQLSTYNDLHLTLKHVHWNVVGPNFIGVHEMIDPQVELVRGYADEVAERIATLGKSPKGTPGAIIKDRTWDDYSVERDTVQAHLAALDLVYNGVIEDTRKSIEKLEDLDLVSQDLLIAHAGELEKFQWFVRAHLESAGGQLTHEGQSTEKGAADKARRKSA
Tag info: N-terminal 6xHis-SUMO-tagged
Expression Region: 1-183aa
Protein length: Full Length
MW: 36.3 kDa
Relevance: Protects DNA from oxidative damage by sequestering intracellular Fe2+ ion and storing it in the form of Fe3+ oxyhydroxide mineral. One hydrogen peroxide oxidizes two Fe2+ ions, which prevents hydroxyl radical production by the Fenton reaction (By similarity). It protects DNA from hydroxyl radical-mediated cleavage. Binds DNA with no apparent sequence specificity without self-aggregation nor promotion of DNA condensation. Is unable to protect DNA from DNase-mediated cleavage.
Reference: "Bimodal protection of DNA by Mycobacterium smegmatis DNA-binding protein from stationary phase cells."Gupta S., Chatterji D.J. Biol. Chem. 278:5235-5241(2003)
Purity: Greater than 90% as determined by SDS-PAGE.
Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.