Recombinant Mouse Tn-I protein (His tag)

SKU: PDEM100261

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:Tn-I

Target Synonym:Troponin I;Tnni3TNNI3Tn-I;Tnni3k

Research Areas:Cardiovascular;Metabolism

Conjugation:

Target Species:Mouse

Expression Host:E.coli

Application:

Fusion tag:N-His

UNIProt ID:P48787

Accession:P48787

Background:Troponin I, also known as TNI and CTF1, is a 24 kDa component of a protein complex on striated muscle thin filaments. Troponin I inhibits the calcium-dependent muscle contraction mediated by Troponins C and T. The expression of cardiac Troponin I (TNNI3) is restricted to cardiac muscle, while TNNI1 and TNNI2 (encoded by distinct genes) are expressed in skeletal muscle. Mutations of cardiac Troponin I are associated with hereditary cardiomyopathy.

Concentration:

Activity:Not validated for activity

Sequence:Met 1-Gly 211

Purity: > 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:23.1 kDa

ObservedMW:32 kDa