Recombinant Mouse TIMP1 Protein (His Tag)

SKU: PDEM100223

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:TIMP1

Target Synonym:Collagenase inhibitor 16C8 fibroblast;EPA;Erythroid-potentiating activity;Metalloproteinase inhibitor 1;TIMP-1;TPA-S1;TPA-induced protein;Timp;Timp-1;Tissue inhibitor of metalloproteinases 1

Research Areas:

Conjugation:

Target Species:Mouse

Expression Host:E.coli

Application:

Fusion tag:N-His

UNIProt ID:P12032

Accession:P12032-1

Background:Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14 (By similarity). Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling.

Concentration:

Activity:Not validated for activity

Sequence:Pro28-Arg205

Purity: > 80% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method.

Calculated MW:19.9 kDa

ObservedMW:22.18 kDa