Recombinant Mouse Olfactomedin-4 (Olfm4), partial

SKU: Q3UZZ4

Size: 100ug. Other sizes are also available.

Activity: Not tested

Research Areas: Cancer

Uniprot ID: Q3UZZ4

Gene Names: Olfm4

Alternative Name(s): (OLM4)

Abbreviation: Recombinant Mouse Olfm4 protein, partial

Organism: Mus musculus (Mouse)

Source: E.coli

Expression Region: 238-500aa

Protein Length: Partial

Tag Info: N-terminal 6xHis-tagged

Target Protein Sequence: CSHGGVVNISAPSVIQLNWLGFSYKYGAWGRDYSPQHPERTLYWVAPLNTDARALEYYRLYDSLDNLLIYSHFRDYRIRYGQGGGTVAFNNNLYVNWYNGGNIAKINLTTNVVDVNRPLPLAAYNNRFSYANVNWQDIDLAVDEQALWAIYATEASTGNIVISKLNDTTLEVISTWVTKQYKPSVSNAFMVCGVLYATRTLNTKTEEIFYYYDTNTEREGNLGITMRKMQERIQSINYHPFDQKLYVYNDGYLLNYDLVFLQT

MW: 36.3 kDa

Purity: Greater than 85% as determined by SDS-PAGE.

Endotoxin: Not test

Biological_Activity:

Form: Liquid or Lyophilized powder

Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance: May promote proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. In myeloid leukemic cell lines, inhibits cell growth and induces cell differentiation and apoptosis. May play a role in the inhibition of EIF4EBP1 phosphorylation/deactivation. Facilitates cell adhesion, most probably through interaction with cell surface lectins and cadherin.

Reference: "Olfactomedin 4 suppresses tumor growth and metastasis of mouse melanoma cells through downregulation of integrin and MMP genes." Park K.S., Kim K.K., Piao Z.H., Kim M.K., Lee H.J., Kim Y.C., Lee K.S., Lee J.H., Kim K.E. Mol Cells 34: 555-561(2012)

Function: