Recombinant Mouse IL6ST/CD130 Protein (His Tag)

SKU: PKSM040841

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: IL6ST/CD130

Target Synonym: 5133400A03Rik;AA389424;BB405851;CD130;D13Ertd699e;gp130

Research Areas:

Conjugation:

Target Species: Mouse

Expression Host: HEK293 Cells

Application: Cell culture;ELISA

Fusion tag: C-His

UNIProt ID: Q00560

Accession: NP_034690.3

Background: Glycoprotein 130 (also known as gp130, IL6ST, IL6-beta or CD130) is a transmembrane protein which is the founding member of the class of all cytokine receptors. CD130/gp130 is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and Oncostatin M (OSM). CD130/gp130 functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. CD130/gp130 plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. A related pseudogene has been identified on chromosome 17. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. CD130/gp130 binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. CD130/gp130 may have a role in embryonic development. The type I OSM receptor is capable of transducing OSM-specific signaling events.

Concentration:

Activity: 1. Measured by its ability to bind mouse IL11Ra in a functional ELISA. 2. Measured by its ability to inhibit the IL6 Rα enhancement of IL6 activity on M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 0.6-3 μg/mL in the presence of 10 ng/mL recombinant human IL6 sR and 20 ng/mL recombinant human IL6.

Sequence: Met 1-Glu 617

Purity: > 97 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 68.0 kDa

ObservedMW: 80-90 kDa