Recombinant Mouse IL-1 Beta protein

SKU: CD00395

Size:2 ug

Background:Interleukin-1beta (IL-1β) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1α and IL-1β binds to the same receptor and has similar but not identical biological properties. The mature mouse IL1β shares 90 % amino acid sequence identity with cotton rat and rat and 65 %-78 % identity with canine, human and rhesus IL1β.

Research_area:Immunology

Purity:> 96 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Mouse Interleukin-1 beta

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by a cell proliferation assay using Mouse D1S cells is less than 2 pg/ml, corresponding to a specific activity of > 5. × 18 IU/mg.

Synonyms:IL-1β, IL-, IL-1beta, Il-1b,IL1B,Mouse

Endotoxin:Less than 1EU/μg of rMuIL-1β as determined by LAL method.

Storage:-20ºC

Sequence:VPIRQLHYRL RDEQQKSLVL SDPYELKALH LNGQNINQQV IFSMSFVQGE PSNDKIPVAL GLKGKNLYLS CVMKDGTPTL QLESVDPKQY PKKKMEKRFV FNKIEVKSKV EFESAEFPNW YISTSQAEHK PVFLGNNSGQ DIIDFTMESV SS

uniprot accession: Q3TI17

Molecular weight:Approximately 17.5 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered solution in PBS, pH 7.4.