Recombinant Mouse FGF-21 protein

SKU: CD00455

Size:5 ug

Background:Fibroblast growth factor-21 (FGF-21) belongs to the large FGF family and it is specifically induced by HMGCS2 activity. In mice, brown adipose tissue becomes a source of systemic FGF21 after cold exposure. FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression) and the activity depends on the presence of KLB. FGF-21, in the presence of betaKlotho as a protein cofactor, signals through the FGFR 1c and 4 receptors. Recombinant Mouse FGF-21 contains 182 amino acids residues and show limited binding to heparin. In addition, Mouse FGF-21 respectively shows 81 % and 92 % a.a. identity to human and rat FGF-21, and it show activity on human and rat cells.

Research_area:Immunology

Purity:> 97 % by SDS-PAGE and HPLC analyses.

Source:Escherichia coli.

Full product name:Recombinant Mouse Fibroblast Growth Factor-21

Biological activity:Fully biologically active when compared to standard. The ED5 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than .5 μg/ml, corresponding to a specific activity of > 2. × 13 IU/mg in the presence of 5 μg/ml of rMuKlotho-β and 1 μg/ml of heparin.

Synonyms:FGF-21, Fgf8c,FGF21,Mouse

Endotoxin:Less than 1 EU/μg of rMuFGF-21 as determined by LAL method.

Storage:-20ºC

Sequence:AYPIPDSSPL LQFGGQVRQR YLYTDDDQDT EAHLEIREDG TVVGAAHRSP ESLLELKALK PGVIQILGVK ASRFLCQQPD GALYGSPHFD PEACSFRELL LEDGYNVYQS EAHGLPLRLP QKDSPNQDAT SWGPVRFLPM PGLLHEPQDQ AGFLPPEPPD VGSSDPLSMV EPLQGRSPSY AS

uniprot accession:Q9JJN1

Molecular weight:Approximately 19.9 kDa, a single non-glycosylated polypeptide chain containing 182 amino acids.

Formulation:

Reconstitution:Lyophilized from a .2 μm filtered concentrated solution in 3 × PBS, pH 7.4.