Recombinant Mouse DKK1 protein (His tag)

SKU: PDMM100011

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:DKK1

Target Synonym:Dickkopf-1;Dickkopf-related protein 1;Dkk-1;Dkk1;mDkk-1

Research Areas:Immunology

Conjugation:

Target Species:Mouse

Expression Host:HEK293 Cells

Application:

Fusion tag:C-His

UNIProt ID:O54908

Accession:O54908

Background:Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity. Plays a role in limb development, attenuates Wnt signaling in the developing limb to allow normal limb patterning.

Concentration:

Activity:Not validated for activity

Sequence:Met1-His272

Purity: > 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin:< 1.0 EU/mg of the protein as determined by the LAL method.

Calculated MW:29.8 kDa

ObservedMW:40 kDa