Recombinant Mouse CXCL1 protein(His Tag)

SKU: PKSM041463

Size: 100μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: CXCL1

Target Synonym: C-X-C Motif Chemokine 1;CXCL1;GRO;GRO-Alpha(1-73);GRO1;GROA;Growth-Regulated Alpha Protein;MGSA;Melanoma Growth Stimulatory Activity;NAP-3;Neutrophil-Activating Protein 3;SCYB1

Research Areas:

Conjugation:

Target Species: Mouse

Expression Host: E.coli

Application: Cell culture

Fusion tag: None

UNIProt ID: P12850

Accession: P12850

Background: Growth-regulated alpha protein (CXCL1,KC), is a member of the alpha chemokine subfamily, was initially identified as an immediate early gene induced in mouse fibroblasts by platelet-derived growth factor. The N-terminal processed form KC(5-72) of the protein is produced by proteolytic cleavage after secretion from bone marrow stromal cells, and shows a highly enhanced hematopoietic activity. Mouse KC shows approximately 63% identity to that of mouse MIP-2. KC is also approximately 60% identical to the human GROs. It has been suggested that mouse KC and MIP-2 are the orthologs of the human GROs and rat CINCs. Cxcl1 has chemotactic activity for neutrophils, and contributes to neutrophil activation during inflammation. Hematoregulatory chemokine, in vitro, suppresses hematopoietic progenitor cell proliferation.

Concentration:

Activity: Measure by its ability to chemoattract BaF3 cells transfected with human CXCR2.The ED₅₀ for this effect is <15 ng/mL.

Sequence: Asn 29-Lys 96

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 50 mM Tris, 150 mM NaCl, pH 8.5.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.

Calculated MW: 7.5 kDa

ObservedMW: 11 kDa