Recombinant Mouse CD39/ENTPD1 Protein (His Tag)

SKU: PKSM041341

Size: 50μg

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation: CD39;ENTPD1

Target Synonym: CD39;Ecto-ATP diphosphohydrolase 1;Ecto-ATPDase 1;Ecto-ATPase 1;Ecto-apyrase;Ectonucleoside triphosphate diphosphohydrolase 1;Lymphoid cell activation antigen;NTPDase 1

Research Areas:

Conjugation:

Target Species: Mouse

Expression Host: HEK293 Cells

Application:

Fusion tag: C-His

UNIProt ID: P55772

Accession: P55772

Background: Ectonucleoside triphosphate diphosphohydrolase-1(NTPDase-1)is an integral membrane protein with an extracellular active site. Recombinant mouse NTPDase-1wasexpressed as a protein lacking its N- andC-terminaltransmembrane domains, resulting in the secretion of the soluble ectodomain. NTPDase-1was originally describedas CD39, a B lymphocyte cell surface marker. but it is also present on the surface of natural killer cells, T cells, and some endothelial cells. NTPDase1hydrolyzes the β-andγ phosphate residues of nucleotides, preferring ATP as the substrate. Through its hydrolysis of extracellular nucleotides, NTPDase-1plays arole in the regulation of purinergic signaling. NTPDase-1is involved in the processes of thromboregulation and vascular inflammation. The administration ofsoluble NTPDase-1may have therapeutic applications for the treatment of some vascular and transplantation-associateddiseases.

Concentration:

Activity: Not validated for activity

Sequence: Thr38-Ile478

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 500mM NaCl, 10% Glycerol, pH 7.4.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 50.5 kDa

ObservedMW: 60-90 kDa