Recombinant Mouse BLMH/BLM Hydrolase Protein (His Tag)

SKU: PKSM040554

Size: 50μg

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Exp date: 12 months

Category ID_II: Recombinant Proteins

Category ID_III: Others

Abbreviation:

Target Synonym: AI035728;Bh;Bmh

Research Areas:

Conjugation:

Target Species: Mouse

Expression Host: E.coli

Application: Enzyme

Fusion tag: N-His

UNIProt ID: Q8R016

Accession: NP_848760.1

Background: The papain superfamily member bleomycin hydrolase (BLMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution. The only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The papain superfamily member bleomycin hydrolase (BLMH) is a neutral cysteine protease with structural similarity to a 20S proteasome. Bleomycin (BLM), a clinically used glycopeptide anticancer agent. BLMH is an essential protectant against BLM-induced death and has an important role in neonatal survival and in maintaining epidermal integrity. Sequencing revealed several putative sites phosphorylated by different types of protein kinases, but no signal sequence, transmembrane domain, N-linked glycosylation site or DNA-binding motif.

Concentration:

Activity: Measured by its ability to hydrolyze Met-AMC. The specific activity is > 500 pmoles/min/μg.

Sequence: Asn 2-Glu 455

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile 50mM Tris, 0.15M NaCl, 10% glycerol, pH 8.0
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: Please contact us for more information.

Calculated MW: 53.3 kDa

ObservedMW: 47 kDa