Recombinant Mouse BGLAP Protein(Trx Tag)

SKU: PDEM100207

Size:100μg

Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping:This product is provided as lyophilized powder which is shipped with ice packs.

Exp date:12 months

Category ID_II:Recombinant Proteins

Category ID_III:Others

Abbreviation:BGLAP

Target Synonym:Bone Gamma-carboxyglutamate Protein;OT;Osteocalcin;BGP;Bglap;Bone Gla protein;Gamma-carboxyglutamic acid-containing protein

Research Areas:Metabolism;Tags and Cell Markers;Developmental Biology;

Conjugation:

Target Species:Mouse

Expression Host:E.coli

Application:

Fusion tag:N-Trx

UNIProt ID:P86546

Accession:P86546

Background:This locus represents naturally occurring read-through transcription between the neighboring PMF1 (polyamine-modulated factor 1) and BGLAP (bone gamma-carboxyglutamate Gla protein) genes on chromosome 1. Alternative splicing results in multiple transcript variants encoding isoforms that share sequence identity with the upstream gene product, but they contain distinct C-termini due to frameshifts versus the downstream gene coding sequence. Recombinant human PMF1-BGLAP protein, fused to His-tag at N-terminus, was expressed in E.coli.

Concentration:

Activity:Not validated for activity

Sequence:Tyr50-Ile95

Purity:> 95% as determined by reducing SDS-PAGE.

Formulation:Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Reconstitution:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Endotoxin:< 10 EU/mg of the protein as determined by the LAL method

Calculated MW:25.1 kDa

ObservedMW:32 kDa